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THE USE OF FROZEN ERYTHROCYTES IN MACROPHAGE STUDIES
Author(s) -
MYHRVOLD VESLEMØY,
JONSEN JON,
MØRLAND BERIT
Publication year - 1983
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1983.tb00007.x
Subject(s) - phagocytosis , cryoprotectant , opsonin , macrophage , receptor , in vitro , chemistry , biology , microbiology and biotechnology , cryopreservation , biochemistry , embryo
Sheep erythrocytes opsonized with IgG or C3b were frozen in various cryoprotective agents, thawed, and compared to corresponding unfrozen erythrocytes exposed to the cryoprotectants and to unfrozen erythrocytes not exposed to the cryoprotectants (controls) as test particles in macrophage attachment and phagocytosis assays. Fc‐receptor‐mediated attachment and phagocytosis were not influenced by the use of any cryoprotective agent tested or by freezing the erythrocytes. This was also the case with C3b‐receptor‐mediated attachment. Phagocytosis via this receptor was negligible in normal macrophages, but tended to be slightly more effective when the test particles had been treated with cryoprotective agents. In vitro stimulation of the macrophages with Escherichia coli endotoxin, however, triggered the capacity to internalize treated and untreated erythrocytes equally.