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THE SURVIVAL OF ANAEROBIC BACTERIA AT 4 °C AND 22 °C ON SWABS IN THREE TRANSPORT SYSTEMS Statistical Evaluation by Application of a Variance Component Model
Author(s) -
JUSTESEN TAGE,
JENSEN ANDERS MØRUP,
HOFFMANN STEEN
Publication year - 1983
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1983.tb00003.x
Subject(s) - bacteroides fragilis , anaerobic exercise , agar , microbiology and biotechnology , agar plate , anaerobic bacteria , eubacterium , biology , bacteria , food science , physiology , genetics , antibiotics
The survival of anaerobic bacteria on swabs in three transport systems at 4 °C and 22 °C was evaluated. The transport systems were a charcoal‐impregnated cotton swab in modified Stuart's transport medium (MS), a plain cotton swab in a dry aerobic tube (DA) and a plain cotton swab in an aerobic agar tube (AA). The test strains were Bacteroides fragilis, B. melaninogenicus ss. intermedius, Fusobacterium necrophorum, Peptostreptococcus anaerobius, Eubacterium lentum and Clostridium ramosum. Inoculated swabs were kept at the described conditions for time periods ranging from 22 1/2 min through 32 d, after which surviving organisms were recovered quantitatively. Results were described by the survival rate, defined as the percentage of organisms initially present, that had survived storage for 24 h. The survival rate was uniformly higher at 4 °C than at 22 °C. In Stuart's transport medium at 4 °C the survival rate was 81.9% compared to 4.3% in dry aerobic tube at 4 °C. At 22 °C the survival rate in dry aerobic tube and aerobic agar tube was 0.003% or less whereas in Stuart's transport medium growth occurred with B. fragilis, E. lentum and C. ramosum.