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EFFECT OF AET TREATMENT OF SHEEP ERYTHROCYTES ON THE ROSETTE TEST IN VARIOUS DISEASES
Author(s) -
Næss Are,
Nyland Harald
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1982.tb01458.x
Subject(s) - mononucleosis , hydrobromide , rosette (schizont appearance) , lymphocyte , medicine , meningitis , immunology , biology , gastroenterology , chemistry , surgery , virus
180 patients with neurological or infectious diseases and 90 normal controls were examined for blood T lymphocyte percentage by the standard method using rosette formation with untreated sheep erythrocytes (E) and a technique using 2‐amino‐ethyl‐thio‐isouronium bromide hydrobromide (AET)‐treated E, with fetal calf serum (FCS) in the medium. Patients with acute meningitis had a decrease and those with infections mononucleosis had an increase in T lymphocyte percentage as measured by both assays. However, patients with active MS and with cerebral tumours had a decrease in T cell percentage by the E but not by the EAET technique. The T lymphocyte percentages of the other 4 patient groups did not differ significantly from those of the controls, regardless of the technique used. In certain diseases, the use or not of AET‐treated indicator cells may thus influence the results of the rosette test.