Premium
CYTOLOGICAL EVENTS IN ALLO‐STIMULATED LYMPHOCYTES TRIGGERED BY EXPOSURE TO ALLOANTIGENS:
Author(s) -
Poulsen Poul Brix,
Nielsen Laust Hangler
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1982.tb01439.x
Subject(s) - lymphoblast , endoplasmic reticulum , mitochondrion , golgi apparatus , glutaraldehyde , lysis , cell , microbiology and biotechnology , incubation , chemistry , biology , cell culture , biochemistry , genetics , chromatography
H‐2 b lymphocytes sensitized against H‐2 d alloantigens or H‐2 k alloantigens were triggered by incubation with disrupted or intact H‐2d target cells. The triggering of the lymphoid cells was stopped by fixation with glutaraldehyde. Changes in cell size, in numerical density of the endoplasmic reticulum (ER), and the Golgi apparatus (GA) as well as in the areal density of the lysosomes and the mitochondria in the lymphoblasts during the first three hours of triggering were measured with a Hewlett Packard digitizer. No significant changes in cell size or in the density of GA, lysosomes and mitochondria were observed after triggering with disrupted target cells, but a significant increase in the density of ER occurred in the lymphoblasts previously stimulated against the target cell. After triggering with intact target cells, cell size of the lymphoblasts increased, as well as the density of GA, mitochondria and lysosomes while the density of ER exhibited a decrease followed by an increase. The disrupted target cells were shown to inhibit the release of 5lCr during lysis of 51 Cr‐labelled target cells.