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CYTOTOXICITY OF TEMPORARILY ADHERENT HUMAN MONONUCLEAR BLOOD CELLS:
Author(s) -
Eggen BjØRn Magne
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1982.tb01428.x
Subject(s) - cytostasis , cytolysis , peripheral blood mononuclear cell , in vitro , cytotoxicity , thymidine , microbiology and biotechnology , cell culture , chemistry , lysis , peripheral blood , monocyte , lytic cycle , biology , immunology , biochemistry , virus , genetics
Monolayers of freshly isolated peripheral blood monocytes are contaminated with 5–10% non‐monocytic cells. These cells detach during the first day of in vitro culture, concomitant with a disappearance of the strong cytostatic and cytolytic activity of freshly isolated monocytes. The detached cells, mostly non‐monocytic cells, exerted cytostasis and cytolysis on the human natural killer (NK) sensitive cell line K‐562. The lytic activity was sensitive to trypsin treatment. The kinetics of cytolysis was NK‐like, with maximal 51 Cr‐release within 4–8 hours of co‐culture, and with a high degree of methyl‐ 3 H‐thymidine release during the first 24 hours of co‐culture. It is suggested that these cells may be a loosely adherent subpopulation of mononuclear cells with NK‐like properties.