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POLYACRYLAMIDE MICROBEADS WITH ENTRAPPED PROTEIN A FROM S. AUREUS FOR CELL SEPARATION
Author(s) -
Lindmark R.,
Larsson E.,
Nilsson K.,
Sjöquist J.
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1982.tb01427.x
Subject(s) - polyacrylamide , trypsin , chromatography , polyacrylamide gel electrophoresis , chemistry , petri dish , electrophoresis , cell , separation method , staphylococcus aureus , bacteria , microbiology and biotechnology , biology , biochemistry , polymer chemistry , enzyme , genetics
A technique was developed for the identification and separation of cells according to their surface antigenic markers, based on immobilization of protein A by entrapment in polyacrylamide microbeads. This method is a further development of the previous techniques with SpA‐containing S. aureus bacteria, but has the advantage of having less unspecific binding to the cells. We show that this technique might be a useful tool for the separation of cells, exemplified by separation of cells on Petri dishes coated with a monolayer of SpA‐microbeads. The viability of separated cells was unaffected and adherent cells were easily removed by trypsin.