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DISSOCIATION BETWEEN TUMOUR CELL CYTOSTATIC AND IMMUNOSUPPRESSIVE CAPACITY OF HUMAN MONOCYTES ACTIVATED WITH LYMPHOKINES
Author(s) -
Unsgaard Geirmund,
HammerstrØM Jens,
Lamvik Jon
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1982.tb01420.x
Subject(s) - lymphokine , monocyte , in vitro , dna synthesis , cell culture , immunology , lymphocyte , mixed lymphocyte reaction , tuberculin , chemistry , cell , microbiology and biotechnology , biology , t cell , immune system , biochemistry , medicine , tuberculosis , pathology , genetics
Activation of monocytes with lymphokines from BCG‐stimulated lymphocytes increased the capacity to inhibit DNA‐synthesis in a tumour cell line (NHIK 3025) at all stages of monocyte maturation in vitro. However, the capacity of monocytes to suppress DNA‐synthesis in mixed lymphocyte cultures (MIX) was reduced, indicating a dissociation of the immuno‐suppressive and tumour cell cytostatic functions of lymphokine‐activated monocytes. Supernatants of lymphokine‐activated monocytes inhibited DNA synthesis in the tumour cell line, but not in MLC. Monocytes from donors with a strong tuberculin reaction produced supernatants with a stronger cytostatic activity than monocytes from donors with a weak tuberculin reaction. By in vitro maturation, even the latter monocytes acquired ability to produce supernatants that were strongly inhibitory to tumour cells.