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IN VITRO STUDIES ON THE TUMOUR CYTOTOXICITY OF NORMAL, STIMULATED AND IMMUNOLOGICALLY ACTIVATED MOUSE MACROPHAGES
Author(s) -
Hansen J. GrØNdahl,
Bennedsen J.,
Rhodes J. M.,
Larsen S. Olesen
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1982.tb01413.x
Subject(s) - lymphokine , cytotoxicity , cytotoxic t cell , lipopolysaccharide , in vitro , macrophage activating factor , microbiology and biotechnology , immunology , cell culture , chemistry , biology , biochemistry , genetics
Peritoneal macrophages (Mø) from mice injected with proteose‐peptone (P), thioglycollate (T) medium or BCG and from mice undergoing a graft versus host (GVH) reaction were tested for their cytotoxicity towards three murine tumour cell lines. The ability of lipopolysaccharide (LPS) and lymphokines to increase cytotoxicity was also studied. TMø were only slightly cytotoxic and this cytotoxicity was not enhanced by LPS and lymphokines. PMø showed the same degree of cytotoxicity as TMø, but this could be enhanced by LPS and lymphokines. Resident macrophages (RMø) sometimes resembled TMø and sometimes PMø. BCGMø were highly cytotoxic and could not be further activated by LPS and lymphokines. GVHMø were moderately cytotoxic but could not be further activated by LPS and lymphokines in contrast to the Mø from hybrid controls.