Premium
FUNCTION OF POLYMORPHONUCLEAR NEUTROPHILIC LEUCOCYTES
Author(s) -
Wandall J. H.
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series c: immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0202
DOI - 10.1111/j.1699-0463.1982.tb01410.x
Subject(s) - chemotaxis , phagocytosis , antibody opsonization , neutrophile , nitroblue tetrazolium , granulocyte , immunology , chemistry , opsonin , microbiology and biotechnology , medicine , inflammation , biology , biochemistry , receptor
The function of polymorphonuclear neutrophils from blood (B‐PMN) and from exudates (E‐PMN) was studied in healthy volunteers. The E‐PMNs were isolated from skin windows with chambers and the chemotactic, phagocytic and Nitro Blue Tetrazolium (NBT) reducing activity measured and compared to that of simultaneously obtained B‐PMNs. The chemotactic and random migration of E‐PMNs in Boyden chambers measured by the leading front and by the chemotactic index were reduced compared with B‐PMNs (p < 0.01). Serum independent phagocytosis of paraffin oil emulsions was significantly increased (p <0.01) by E‐PMNs after 12, 24 and 48 hours and not correlated to the function of B‐PMNs. Opsonization with autologous serum increased the phagocytosis by both E‐PMNs and B‐PMNs, but mainly the latter. The NBT reduction by E‐PMNs was increased (p < 0.01) and positively correlated to the NBT reduction by resting B‐PMNs. In contrast, there was no difference in NBT reduction between phagocytosing E‐PMNs and B‐PMNs. Mobilization of PMNs to an inflammatory focus in healthy subjects induces marked changes in the function, and B‐PMN function cannot always be assumed to reflect the function of PMNs from inflammatory sites.