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DETECTION OF CLOSTRIDIUM PERFRINGENS TYPE A ENTEROTOXIN BY ELISA
Author(s) -
OLSVIK øRJAN,
GRANUM PER EINAR,
BERDAL BJØRN P.
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1982.tb00144.x
Subject(s) - enterotoxin , clostridium perfringens , microbiology and biotechnology , feces , chemistry , food science , toxin , chromatography , biology , bacteria , escherichia coli , biochemistry , genetics , gene
A four‐layer sandwich ELISA was developed for the detection of Clostridium perfringens enterotoxin. This ELISA method was sensitive down to 0.1 ng/ml of purified enterotoxin in trypticase soy broth, and 1.0 ng/ml of enterotoxin in heterogeneous solutions such as meat products and faeces.