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EFFECT OF BETA ‐INTERFERON ON IN VITRO SPREADING OF MOUSE PERITONEAL MACROPHAGES
Author(s) -
ROLLAG HALVOR,
DEGREÉ MIKLOS
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1982.tb00136.x
Subject(s) - in vitro , newcastle disease , beta (programming language) , interferon , andrology , immunology , chemistry , virus , medicine , biochemistry , computer science , programming language
The influence of a homologous toa‐interferon preparation (MuIFN‐β) on in vitro adherence and the early and late phase of spreading of mouse peritoneal macrophages (MPM) was tested. MPM from mice pre‐treated with 2 × 10 4 U of MuIFN‐β did not differ in ability to adhere to a glass surface, compared to controls. However, when the early phase of MPM spreading was tested, the percentage of spreading cells from mice injected with MuIFN‐β or Newcastle disease virus was almost doubled as compared to MPM from control mice. To assay the late phase of MPM spreading, MPM from unstimulated mice were seeded on glass surfaces and cultivated with or without MuIFN‐β for 1‐24 hours. MuIFN‐β enhanced the spreading of MPM with maximal difference 3‐5 hours after seeding. The effect was dose‐dependent up to 102U per ml of MuIFN‐β The effect was also species ‐ specific and neutralized by addition of anti‐MuIFN‐β globulin. Morphometric measurements of the spreading of MPM indicated that after 24 hours of MuIFN‐β treatment the mean area occupied was 50 per cent larger than that of controls.