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THIN LAYER ISO‐ELECTRO FOCUSING OF INTRACELLULAR BACTERIAL PROTEIN IN CLASSIFICATION OF SOME REPRESENTATIVES OF THE FAMILIES ENTEROBACTERIACEAE AND VIBRIONACEAE
Author(s) -
GJERDE JAN
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1982.tb00135.x
Subject(s) - enterobacteriaceae , intracellular , vibrionaceae , biology , polyacrylamide gel electrophoresis , bacteria , biochemistry , genetics , escherichia coli , gene , enzyme
Intracellular bacterial protein from some representatives of the families Enlerobacteriaceae and Vibrionaceae has been investigated using thin layer iso‐electric focusing in polyacrylamide gel. A marked difference between the protein patterns was observed between species within the same family. Carrying out the iso‐electric focusing at pH range 3.5‐9 gave a large number of protein bands. Increased resolution of the protein bands was obtained using iso‐electric focusing at the pH range 4–6.S. Careful standardization of sample preparation, application and running conditions gave good reproducibility of intracellular proteins from bacterial subcultures.