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AN INCN PLASMID EXHIBITING A HIGH FREQUENCY OF TRANSFER IN BROTH MEDIA
Author(s) -
Jørgensen N. H. Feilberg,
Stenderup A.
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1982.tb00125.x
Subject(s) - pilus , plasmid , bacterial conjugation , mutant , agar , microbiology and biotechnology , biology , strain (injury) , genetics , bacteria , escherichia coli , gene , anatomy
A mutant of the IncN plasmid N3T was isolated. This mutant (pNJ101) exhibits equal transfer frequencies in broth and on agar. IncN plasmids have rigid pili and do generally transfer at much lower frequencies in broth than on agar, hypothetically explained as the vulnerability of the pili towards convection forces in liquid. Strains with the mutant do have rigid pili, so other mechanisms regulating IncN plasmid transfer must be sought.