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THE USE OF FROZEN ERYTHROCYTES IN MACROPHAGE STUDIES
Author(s) -
MYHRVOLD VESLEMØY,
JONSEN JON,
MØRLAND BERJT
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1982.tb00122.x
Subject(s) - cryoprotectant , macrophage , in vitro , cryopreservation , andrology , blood preservation , chemistry , red blood cell , biology , biochemistry , immunology , microbiology and biotechnology , medicine , embryo
The attachment of red cells to mouse peritoneal macrophages in vitro was tested with erythrocytes (from sheep and man) which had been subjected to different cryoprotective agents and freezing procedures. The experiments showed that with dimethylsulfoxide (DMSO) as the cryoprotective agent no difference in macrophage attachment was seen whether the erythrocytes were frozen or not. With the other cryoprotectants tested, macrophages were more efficient in attaching frozen than unfrozen erythrocytes. This was the case with erythrocytes from both sheep and man. Similar results were obtained with fresh (one week) and old (two weeks) erythrocytes.