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IMMUNOCHEMICAL STUDIES OF PARTIALLY HYDROLYZED LIPOPOLYSACCHARIDE FROM FUSOBACTERIUM NUCLEATUM ATCC 10953
Author(s) -
HOFSTAD TOR
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1982.tb00120.x
Subject(s) - fusobacterium nucleatum , hydrolysis , size exclusion chromatography , polysaccharide , chemistry , fraction (chemistry) , galactose , heptose , acid hydrolysis , chromatography , elution , biochemistry , acetic acid , glucosamine , molecular mass , mannose , hexosamines , bacteria , biology , enzyme , genetics , porphyromonas gingivalis , mutant , gene
Fusobacierium nucleatum ATCC 10953 LPS was split by hydrolysis with 1 per cent acetic acid into acid‐soluble polysaccharide and lipid A. Gel filtration of the acid‐soluble polysaccharide on Bio‐Gel P‐60 gave a high‐molecular‐weight fraction eluted with the void volume (V 0 ), and a fraction eluted at 2.4 × V o . The high‐molecular‐weight fraction was serologically active, and contained glucose as the only sugar. The other fraction, which was serologically inactive, contained L‐ glycero ‐D‐ manno ‐hepioss, galactose, glucosamine, keto‐deoxy‐octonate, and phosphorus. The fraction eluted at 2.4 × V o is thought to constitute the polysaccharide core region, whereas the high‐molecular‐weight fraction may represent O‐antigenic side chains.