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PROPERTIES OF THE SERUM FACTOR INVOLVED IN THE PRECIPITATION REACTION WITH STAPHYLOCOCCUS CAPITIS
Author(s) -
OSLAND ARVE
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1982.tb00105.x
Subject(s) - isoelectric point , chromatography , chemistry , agarose , elution , isoelectric focusing , immunoelectrophoresis , ultracentrifuge , precipitation , ammonium , biochemistry , organic chemistry , antibody , biology , immunology , physics , meteorology , enzyme
The serum factor was precipitated by both Rivanol® and ammonium sulphate, and it bound to both DEAE‐cellulose and Blue‐Sepharose CL‐6B from which it subsequently could be eluted. Immunoelectrophoresis showed that the serum factor had ai‐mobility, and isoelectric focusing indicated an apparent isoelectric point of 5.2. Purification by ultracentrifugation, followed by agarose chromatography, showed that the serum factor is present in the high density lipoprotein fraction of serum.