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EFFECTS OF NICOTINE ON ENDOCYTOSIS AND INTRACELLULAR DEGRADATION OF HORSERADISH PEROXIDASE IN CULTIVATED MOUSE PERITONEAL MACROPHAGES
Author(s) -
THYBERG JOHAN,
NILSSON JAN
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series a :pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0164
DOI - 10.1111/j.1699-0463.1982.tb00098_90a.x
Subject(s) - horseradish peroxidase , nicotine , intracellular , phagocytosis , endocytosis , chemistry , peroxidase , in vitro , vesicle , biochemistry , enzyme , pharmacology , microbiology and biotechnology , biology , medicine , cell , membrane
Thioglycollate‐elicited mouse peritoneal macrophages were cultivated in vitro in control medium or medium containing nicotine (1 nM‐1 μM). The drug caused a moderate lysosomal vacuolation and formation of vesicles with a dense core or ring. Furthermore, it partially inhibited uptake and intracellular degradation of horseradish peroxidase without affecting the specific activities of the lysosomal enzymes β‐glucuronidase and β‐N‐acetylglucosaminidase in lysates of the cells. A slight inhibitory effect on the phagocytosis of latex beads was also noted. Since nicotine was used in concentrations of the same magnitude that may be obtained in the blood after smoking one cigarette, it seems worthwhile to further explore these findings in relation to the medical risks of smoking.