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DETECTION OF MEASLES IgM ANTIBODIES BY ENZYME‐LINKED IMMUNOSORBENT ASSAY (ELISA)
Author(s) -
PEDERSEN IB RODE,
ANTONSDOTTIR AUDUR,
EVALD TORBEN,
MORDHORST CARL H.
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1982.tb00098.x
Subject(s) - antibody , virology , measles , enzyme , measles virus , microbiology and biotechnology , biology , immunology , vaccination , biochemistry
An enzyme‐linked immunosorbent assay (ELISA) for the detection of IgM antibodies in serum during acute or recent measles infections is described. False positive reactions due to rheumatoid factors (RF) were not observed in the test of human sera to which were added sera containing RF. Monospecific antisera against the haemagglutinin, nucleoprotein and polymerase of measles virus were used in a blocking IgM ELISA, revealing a sensitive method for the detection of the antibodies that take part in the immune reactions. An ELISA for the detection of measles‐specific IgG in order to reveal IgM or IgG antibodies in the human sera is also described.