Premium
METHOD FOR ISOLATION OF GARDNERELLA VAGINALIS (HAEMOPHILUS VAGINALIS) Characterization of Isolates by Gas Chromatography
Author(s) -
CSÁNGÓ PÉTER A.,
HAGEN NORMAN,
JAGARS GARLETTA
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series b: microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0180
DOI - 10.1111/j.1699-0463.1982.tb00087.x
Subject(s) - gardnerella vaginalis , haemophilus , microbiology and biotechnology , isolation (microbiology) , hemolysis , fatty acid , bacteria , biology , chromatography , chemistry , biochemistry , bacterial vaginosis , genetics , immunology
A method for the isolation of Gardnerella vaginalis (Haemophilus vaginalis) is presented. Bacteria isolated from 48‐hour cultures grown on human blood agar were identified by means of β‐hemolysis, colony morphology, sensitivity to antimicrobial agents, oxydase and catalase reactions. Thirty‐eight clinical isolates and one test strain were examined for fatty acid composition. Hexadecanoic (16:0), octadecenoic (18:1) and octadecanoic (18:0) were the major fatty acids. Also present, but in minor quantities, were myristic (14:0), hexadecenoic (16:1) and octadecadienoic (18:2) acids. Only insignificant differences between isolates could be detected. No hydroxy fatty acids commonly found in gram‐negative bacteria were encountered. Gas chromatographic analysis of G. vaginalis revealed a characteristic and relatively simple pattern. The results support the use of the isolation method, which provides conditions highly selective for G. vaginalis.