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IMMUNO‐HISTOCHEMICAL LOCALIZATION OF MYOGLOBIN IN HUMAN MUSCLE TISSUE AND EMBRYONAL AND ALVEOLAR RHABDOMYOSARCOMA
Author(s) -
KINDBLOM LARSGUNNAR,
SEIDAL TOMAS,
KARLSSON KARIN
Publication year - 1982
Publication title -
acta pathologica microbiologica scandinavica series a :pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0108-0164
DOI - 10.1111/j.1699-0463.1982.tb00078_90a.x
Subject(s) - fixative , staining , paraformaldehyde , pathology , immunoperoxidase , myoglobin , fixation (population genetics) , embryonal rhabdomyosarcoma , skeletal muscle , biology , immunohistochemistry , anatomy , chemistry , rhabdomyosarcoma , sarcoma , medicine , biochemistry , immunology , antibody , gene , monoclonal antibody
An immuno‐histochemical investigation of the presence and localization of myoglobin was performed on cardiac and skeletal muscle tissue and on 9 embryonal and 9 alveolar rhabdomyosarcomas, utilizing an immunoperoxidase technique. Cardiac muscle fibres were evenly stained whereas staining of skeletal muscle fibres varied, giving a mosaic‐like pattern. Of the fixatives used (4 percent formaldehyde, 4 percent paraformaldehyde, formaldehyde‐glutaraldehyde, and Bouin's fixative), 4 percent formaldehyde gave the most prominent staining; short fixation‐time slightly increased the staining intensity. All the 9 embryonal and 9 alveolar rhabdomyosarcomas contained positively‐stained tumour cells. The number of cells positively‐stained and the intensity of the staining varied with differentiation: the most differentiated rhabdomyoblasts stained most intensely. However, also many poorly‐differentiated tumour cells in highly cellular areas were positive. Myoglobin is considered a suitable marker of rhabdomyoblastic differentiation, thus the method used in this study may be valuable in the diagnosis.