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INHIBITORY EFFECT ON TUMOUR COLONY FORMATION OF MOUSE SERUM ASSOCIATED WITH TUMOUR RESISTANCE IN VIVO IN SEMI SYNGENEIC MICE
Author(s) -
GUNDERSEN STEIN,
WIBE EINAR,
FUNDERUD STEINAR,
GRZENLAKPUCZYINSKA IRENA,
GODAL TORE
Publication year - 1981
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1981.tb02691.x
Subject(s) - in vivo , in vitro , biology , lewis lung carcinoma , neoplasm , melanoma , microbiology and biotechnology , cancer research , immunology , chemistry , cancer , biochemistry , metastasis , genetics
Differences in tumour susceptibility between strains of mice (C57B1/6 and C57B1/6 × DBA/2 = B 6 D 2 F 1 ) could be demonstrated for several tumours of C57B1 origin, both solid tumours (B 16 melanoma and Lewis lung carcinoma) and lymphomas (RBL‐5, 136‐3 and ALC). Serum from mice with high tumour resistance in vivo (B 6 D 2 F 1 ) showed an inhibitory effect on tumour colony formation in a soft agar colony assay. Serum from mice with lower tumour resistance (C57B1/6) had no effect. When other F 1 hybrids of C57B1/6 parental origin were tested, the same correlation between in vitro inhibition of tumour colony formation and in vivo susceptibility was found. The serum factor was species non‐specific, since the activity was expressed against in vitro grown cell lines of human origin. The tumour colony inhibitory activity was heat sensitive (56°C for 30 minutes), precipitable by (NH 4 )2SO 4 , and not removed by adsorption on tumour cells. These results demonstrate the existance of a naturally‐ocurring humoral tumerostatic factort(s) which correlates to in vivo susceptibility to tumour cells. Its relationship to NK cell activity is discussed.