Premium
METHODOLOGICAL APPROACHES TO ANTIGEN IDENTIFICATION IN SOLUBLE IMMUNE COMPLEXES; A MODEL STUDY
Author(s) -
HUSBY S.,
SVEHAG S. E.,
NIELSEN H.,
HØIBY N.,
SCHIØTZ P. O.
Publication year - 1981
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1981.tb02679.x
Subject(s) - antigen , immunoelectrophoresis , chemistry , immunoradiometric assay , peg ratio , immune complex , immune system , chromatography , antibody , antigen antibody complex , biochemistry , immunology , radioimmunoassay , biology , finance , economics
The usefulness of different techniques for identification of immune complex‐(IC) bound antigen(s) was investigated in two Ab‐Ag model systems. The following approaches were used: 1) determination of IC‐activity before and after removal of IC by adsorption to antibody‐specific solid phase systems, 2) analysis of PEG‐insoluble IC by reference line‐rocket Immunoelectrophoresis and 3) reprecipitation of PEG‐insoluble IC in the presence of 125 I‐labelled antibody to suspected antigens and subsequent analysis of the distribution of the radioactivity. This latter approach, the use of a double PEG precipitation immunoradiometric assay, was found most useful and simple for identification of antigen(s) contained in soluble IC. The technique is most sensitive in demonstrating IC‐bound antigen(s) when the IC are formed in slight to moderate antigen excess.