Premium
CULTURED HUMAN SYNCYTIOTROPHOBLASTS SYNTHESIZE PREGNANCY‐SPECIFIC BETA‐1‐GLYCOPROTEIN (SP 1 )
Author(s) -
HEIKINHEIMO M.,
SAKSELA O.,
LEHTOVIRTA P.,
SEPPÄLÄ M.,
BOHN H.
Publication year - 1981
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1981.tb02677.x
Subject(s) - syncytiotrophoblasts , syncytiotrophoblast , immunoprecipitation , glycoprotein , microbiology and biotechnology , biology , cell culture , placenta , pregnancy , chemistry , fetus , genetics
The synthesis of pregnancy‐specific beta‐1‐glycoprotein (SP 1 ) was studied in human syncytiotrophoblast cultures from first trimester placentas. Incorporation of 35 S‐methionine into SP 1 was demonstrated by immunoprecipitation and polyacrylamide gel electrophoresis. In double diffusion the precipitation line of neosynthesized SP 1 gave a reaction of complete immunological identity with maternal serum SP 1 . By indirect immunofluorescence intracellular SP 1 was localised in the perinuclear region of the cells. The average rate of SP 1 release into culture fluid was 600 ng/mg protein in 24 hours. On a molar basis the release of SP 1 was 10 times less than that of human chorionic gonadotrophin (hCG). Our results demonstrate, for the first time, that cultured human syncytiotrophoblasts synthesise SP 1 immunologically indistinguishable from placental SP 1 .