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A »REVERSE« SOLID‐PHASE RADIO‐IMMUNOASSAY FOR IgM‐ANTIBODIES TO HEPATITIS A VIRUS
Author(s) -
MEURMAN OLLI H.,
MATTER LUKAS,
KRISHNA RANGACHAR V.,
KRECH ULRICH H.
Publication year - 1981
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1981.tb02668.x
Subject(s) - antibody , antigen , immunoglobulin m , rheumatoid factor , virology , immunoassay , hepatitis a , hepatitis a virus , titer , virus , hepatitis , immunology , medicine , immunoglobulin g
A »reverse« solid‐phase radio‐immuno‐assay for IgM antibodies to hepatitis A virus (HAV) was developed. Anti‐human IgM immunoglobulins were bound on the wells of polyvinylchloride microliter plates. Serum specimens were incubated in the anti‐human IgM coated wells and bound IgM antibodies were then assayed for antigen specificity by subsequent incubations with HAV antigen and 125 I‐labelled human anti‐HAV IgG. The test showed a high sensitivity and specificity for anti‐HAV IgM antibodies. No false‐positive reactions were observed either in the sera from patients with hepatobiliary disorders other than HAV infection or in the sera containing both rheumatoid factor and anti‐HAV IgG antibodies. In acute HAV infections specific IgM antibodies were present already in the first specimens taken within a few days after the onset of jaundice. The persistence of the IgM antibodies was from 4 to 6 months. IgM antibody titers up to 1,000,000 were observed in the acute phase of HAV infection. In routine diagnostic work the titration of the sera was not necessary, since a reliable qualitative result was obtained by testing the sera in a single diluton of 1:100. A similar »reverse« immuno‐assay principle may be adaptable for the diagnostic determination of IgM antibodies to different viral and microbial antigens.