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IDENTIFICATION OF LOW LEVELS OF HEAT LABILE ENTEROTOXIN IN ESCHERICHIA COLI FROM CHILDREN WITH DIARRHOEA
Author(s) -
OTNÆSS ANNEBRIT,
HALVORSEN SVERRE
Publication year - 1981
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1981.tb00172_89b.x
Subject(s) - enterotoxin , heat labile enterotoxin , toxin , escherichia coli , microbiology and biotechnology , feces , strain (injury) , biology , chemistry , food science , biochemistry , gene , anatomy
Out of 40 children with gastroenteritis and massive growth of E. coli in fæces, 7 yielded growth of E. coli strains producing heat‐labile enterotoxin (LT), as identified by enzyme‐linked immunosorbent assay (ELISA) using anticholera toxin coated plates or ganglioside coated plates. The toxin production in vitro was low, and decreased upon subculturing for 3 months in the laboratory. Only two of the strains were identified as LT‐positive by the YI adrenal cell test. In addition, an LT‐producing strain was isolated from an adult who had recently returned from Jordan. Sonication of the strains after subculturing released cell‐bound LT. The clinical importance of such low toxin producing E. coli strains is not known.