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DETECTION OF YERSINIA ENTEROCOLITICA O‐ANTIGENS BY DOUBLE DIFFUSION IN AGAR AND CHEMICAL CHARACTERIZATION OF LIPOPOLYSACCHARIDES
Author(s) -
DAGESTAD KARI,
GROV ARNE,
OEDING PER
Publication year - 1981
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1981.tb00157_89b.x
Subject(s) - yersinia enterocolitica , microbiology and biotechnology , agar , antigen , agar gel , yersinia , chemistry , biology , bacteria , immunology , genetics
Double diffusion in agar showed that Yesinia emerocolitica O‐serotypes 3, 7, 8 and 9 each contained a type‐specific precipitinogen. Serotype 6 contained several specific precipitinogens and was heterogeneous. The type‐specific precipitinogens were shown to be lipopolysaccharides (LPS). Acid hydrolysis of the isolated LPS followed by gel filtration separated the material into two peaks, the first of which contained the serologically active fractions. The active fraction of serotype 3 contained relatively large amounts of a terminally linked deoxyhexose, probably 6‐deoxy‐L‐altrose. Fucose was found to be present in serotype 7.