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PURIFICATION AND CHARACTERIZATION OF ALEUTIAN DISEASE VIRUS
Author(s) -
Aasted Bent
Publication year - 1980
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1980.tb02650.x
Subject(s) - mink , virus , isoelectric focusing , immunoelectrophoresis , capsid , virology , antibody , agarose , microbiology and biotechnology , biology , polyacrylamide gel electrophoresis , parvovirus , chemistry , biochemistry , enzyme , immunology , ecology
Virus was isolated from infected mink organs by a combination of tissue homogenization, fluorocarbon extraction and ultracentrifugation. The final preparation was analysed by crossed immunoelectrophoresis and electronmicroscopy. Virions had a capsid diameter of 22 nm. Preparative agarose electrophoresis separated virions from contaminating ferritin. Crossed immunoelectrophoresis of virus gave a single precipitate with sera from infected mink. Crossed immunoelectrophoretic analysis with intermediate gels showed that a part of the virus preparation was complexed with antibody. Serum from a certain mink was found to contain precipitating antibody to (poly)nucleotid. Virus and virus‐antibody complexes were found to focus at pH 4.0–4.4 in isoelectric focusing. In SDS‐polyacrylamide gel‐electrophoresis the main virus protein was found to have a molecular weight of 69000. This study gives further support to the classification of aleutian disease virus as a parvovirus.