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AN ANTIGEN COMMON TO A WIDE RANGE OF BACTERIA.
Author(s) -
Sompolinsky David,
Hertz Jesper B.,
HøIby Niels,
Jensen Klaus,
Mansa Bendt,
Samra Zemira
Publication year - 1980
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1980.tb02620.x
Subject(s) - antigen , sephadex , chromatography , size exclusion chromatography , chemistry , immunoelectrophoresis , sodium dodecyl sulfate , polyacrylamide gel electrophoresis , agarose , fractionation , bacteria , antibody , gel electrophoresis , sodium , microbiology and biotechnology , biochemistry , biology , immunology , enzyme , genetics , organic chemistry
In crude water‐soluble extracts of Pseudomonas aeruginosa 64 antigens can be demonstrated by crossed immunoelectrophoresis in agarose with polyvalent Pseudomonas‐immunoglobulin. One of these antigens cross‐reacts with antigens prepared from bacteria of a wide range of taxonomic groups. Monospecific immunoglobulins to this antigen (Common Antigen) were produced by immunization with the appropriate immunocomplex extracted from agarose. Common Antigen was purified by the combination of two fractionation methods: Precipitation of the crude extract with 18% (w/v) sodium sulfate, followed by gel filtration of the supernatant on a Sephadex G‐200 column. By this method, 35% of Common Antigen from the crude extract was recovered, more than half of the fractions electrophoretically pure. Electrophoresis of reduced Common Antigen on a dodecyl sodium sulfate polyacrylamide gel revealed two protein bands with apparent molecular weights of 59–62 000 and 62–65 000, respectively. The untreated antigen, however, passed a column of Sephadex G‐200 with the void volumen, indicating a substance of high molecular weight (> 4–600 000).