Premium
AN EFFECTIVE, SELECTIVE MEDIUM FOR YERSINIA ENTEROCOLITICA CONTAINING SODIUM OXALATE
Author(s) -
Soltész Lészló V.,
Schalén Claës,
Märdh PerAnders
Publication year - 1980
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1980.tb02598.x
Subject(s) - yersinia enterocolitica , lactose , agar , food science , oxalate , enterobacteriaceae , microbiology and biotechnology , chemistry , growth medium , yersinia , bacteria , sucrose , fermentation , sodium oxalate , biology , biochemistry , escherichia coli , genetics , organic chemistry , gene
A medium (»Y« medium) is described, which was more efficient for the isolation of Yersinia enterocolitica from experimentally infected faecal specimens than desoxycholate‐citrate, McConkey, lactose‐sucrose‐urea (LSU) agar, and Yersinia selective medium (Wauter's medium). The »Y« medium consists of casein hydrolysate and peptone serving as carbon and energy sources. A high selectivity is achieved by its contents of sodium oxalate and bile salts. The oxalate suppresses growth of gramnegative rods, including members of the family Enterobacteriaceae and of Pseudomonas spp., while the bile salts inhibit growth of gram‐positive bacteria. In the few instances coliform rods grew on the »Y« medium, they could easily be distinguished by their fermentation of lactose, included in the medium, and the fact that colonies of organisms were surrounded by an opaque zone of precipitated bile salts. The most optimal condition for the isolation of Y. enterocolitica from stools was achieved at incubation of the »Y« medium at 29 °C for 2 days.