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ENZYMECYTOCHEMISTRY AND IMMUNOHISTOCHEMISTRY IN MONOCLONAL GAMMOPATHY AND REACTIVE PLASMACYTOSIS
Author(s) -
JOHANSEN PREBEN,
JENSEN MOGENS KROGH
Publication year - 1980
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1980.tb02509.x
Subject(s) - plasmacytosis , multiple myeloma , pathology , immunoperoxidase , medicine , bone marrow , monoclonal gammopathy of undetermined significance , monoclonal , immunohistochemistry , antibody , monoclonal antibody , immunology
Peripheral blood smears and bone‐marrow smears from 29 patients with malignant M‐components (25 with multiple myeloma and 4 with malignant lymphoma), 13 patients with benign monoclonal gammopathy (BMG), and 20 patients with polyclonal reactive plasmacytosis were examined by leucocyte alkaline phosphatase score (LAP‐score) and by acid phosphatase score in plasma cells from bone‐marrow smears. Furthermore, tissue sections from marrow biopsies from all patients were examined by the three‐layer unlabelled immunoperoxidase technique to detect cytoplasmic immunoglobulin. The LAP‐score was significantly higher in patients with malignant M‐components than in patients with BMG and also higher in IgA and IgG myeloma than in IgA and IgG BMG, but the latter difference was not significant. Furthermore, a significant positive correlation between paraprotein concentration and LAP‐score was found in multiple myeloma. Acid phosphatase score in plasma cells showed no clear distinction between multiple myeloma and BMG. Immunohistochemical examination showed a distinct monoclonal pattern in both multiple myeloma and BMG, allowing identification of the M‐cxsomponent which in all cases corresponded to the M‐component detected by serum examination. Cells producing immunoglobulin classes not matching the M‐component were more rare in multiple myeloma than in BMG, but the difference between the two conditions was quantitative and allowed no clear distinction.