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QUANTITATIVE STUDIES OF THE GASTRIN‐PRODUCING CELLS OF THE HUMAN ANTRUM
Author(s) -
NIELSEN H. OVERGAARD,
HALKEN S.,
LORENTZEN M.
Publication year - 1980
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1980.tb02494.x
Subject(s) - antrum , gastrin , immunoperoxidase , gastric antrum , pyloric antrum , biopsy , population , medicine , pathology , biology , stomach , immunology , antibody , monoclonal antibody , environmental health , secretion
The antral gastrin‐producing cells (G‐cells) have been identified by the indirect immunoperoxidase technique in two antrum preparations removed due to a recurrent duodenal and gastric ulcer. Morphometric principles were applied to the G‐cells with determination of their volume density, numerical density, and mean cell volume. The study showed that within‐observer variation, between‐observer variation and within‐patient variation were negligible, provided at least 200 G‐cells were counted. A biopsy material can be used, as well as larger tissue blocks, when this minimum sample size is respected. A method for estimating the total G‐cell population and the total G‐cell volume in the antrum was developed. In the antrum removed due to a gastric ulcer the number of G‐cells was 190 times 10 6 and their total volume 176 mm 3 .