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CYTOPLASMIC EFFECTS OF X‐IRRADIATION ON CULTURED CELLS IN A NON‐DIVIDING STAGE
Author(s) -
HAMBERG HANS,
BRUNK ULF T.,
ERICSSON JAN L. E.,
JUNG BO
Publication year - 1980
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1980.tb02491.x
Subject(s) - microfilament , cytoplasm , irradiation , microtubule , motility , interphase , biology , intermediate filament , tubulin , antibody , cytoskeleton , cell , immunofluorescence , microbiology and biotechnology , biophysics , chemistry , immunology , biochemistry , physics , nuclear physics
Cultured human glial cells were blocked in interphase (G 1 ) by 24 h of serum starvation and subsequently subjected to 200 Gy, 8 MV X‐radiation. Immediately following irradiation the cultures were transferred to serum‐containing medium. Time‐lapse cinemicrography performed during the next 48 h showed a profoundly disturbed motility with decreased ability for polarization and locomotion of the irradiated cells. Specimens fixed 24 and 48 h after irradiation and investigated by immunofluorescence with tubulin‐antibodies and DNase/DNase‐antibodies, and by whole cell transmission electron microscopy showed derangements in the distribution of microfilament bundles related to the cytoplasmic ramification of the irradiated cells, but otherwise no detectable alterations in structure or distribution of either microtubules or microfilaments. It is suggested that the alteration in the arrangement of filament bundles is of importance to the impaired locomotion of the irradiated cells.