SPECIFIC DETECTION OF DIFFERENT PLASMA PROTEINS IN GEL FILTRATION FRACTIONS BY SOLID PHASE ENZYMEIMMUNOASSAY (EIA) | Zendy

VILJANEN MATTI K. | Zendy

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SPECIFIC DETECTION OF DIFFERENT PLASMA PROTEINS IN GEL FILTRATION FRACTIONS BY SOLID PHASE ENZYMEIMMUNOASSAY (EIA)

Author(s) -

VILJANEN MATTI K.

Publication year - 1980

Publication title -

acta pathologica microbiologica scandinavica section c immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.909

H-Index - 88

eISSN - 1600-0463

pISSN - 0304-1328

DOI - 10.1111/j.1699-0463.1980.tb00087.x

Subject(s) - chromatography , antiserum , size exclusion chromatography , fractionation , chemistry , alkaline phosphatase , cell fractionation , antibody , biochemistry , membrane , biology , enzyme , immunology

An enzymeimmunoassay (EIA) is described enabling sensitive and specific immunodetection of different proteins in analytical and preparative fractionation of biological mixtures. The method is based on the ability of polystyrene to bind proteins and polypeptides. Commercially available rabbit antisera are used combined with the use of commercial alkaline phosphatase conjugated anti‐rabbit‐IgG antibodies. The good assortment of rabbit antisera available makes possible identification of hundreds of proteins in biological samples by the present method.

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