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CHARACTERIZATION OF THE BACTERICIDAL ANTIBODY RESPONSE AGAINST HAEMOPHILUS INFLUENZAE
Author(s) -
DAHLBERG TERESA,
BRANEFORS PAULA
Publication year - 1980
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1980.tb00082.x
Subject(s) - hyperimmunization , haemophilus influenzae , microbiology and biotechnology , antibody , bacteria , antibody response , complement fixation test , immunization , titer , immune system , chemistry , biology , immunology , antibiotics , serology , genetics
Rabbits were used in a study of the bactericidal (BC) antibody response against capsulated Haemophilus influenzae of types a and b and their respective non‐capsulated variant strains. Previously described methods, complement fixation and indirect hemagglutination, were used for comparison. A bactericidal antibody response was demonstrable within a week after the primary immunization against capsulated as well as non‐capsulated bacteria. After the hyperimmunization course the BC titres against non‐capsulated bacteria were about 1:4,000, while the BC titres against the capsulated – types a and b–strains reached levels of no less than 1:130,000. Samples of separated hyperimmune sera showed BC activity in IgM‐ as well as IgG‐containing fractions, whereas the BC activity of primary response samples was demonstrable mainly in IgM‐containing fractions. The BC effect on non‐capsulated bacteria of primary response samples were more heat labile than those obtained after hyperimmunization. In immune sera a crossreactive BC activity was demonstrable on non‐capsulated bacteria, the titre being 32–64‐fold lower than that of the homologous activity.