Premium
DEGRADATION OF NON‐OPSONIZED 32 P‐LABELLED E. COLI BY UNSTIMULATED MOUSE PERITONEAL MACROPHAGES
Author(s) -
ROLLAG HALVOR
Publication year - 1980
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1980.tb00081.x
Subject(s) - opsonin , bacteria , microbiology and biotechnology , macrophage , in vitro , ingestion , escherichia coli , biology , chemistry , phagocytosis , immunology , endocrinology , biochemistry , genetics , gene
Five hours after the ingestion of non‐opsonized E. coli by mouse peritoneal macrophages in vitro. 60–70 per cent of the bacteria were killed, estimated as decrease in colony forming units. When employing 32 P‐labelled E. coli , 50–60 per cent of the radioactivity was released to the medium by the macrophages during the same period of time. The mean number of visually‐counted bacteria per macrophage was constant in the post‐ingestive period. The macrophages killed the bacteria without early destruction. The release of radioactivity seemed to reflect the degradation of bacteria in the macrophages.