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THE CELLULAR REACTION IN NORMAL AND TUMOUR‐BEARING MICE FOLLOWING INTRAPERITONEAL GLUCAN INJECTION
Author(s) -
OLSTAD R.,
SELJELID R.
Publication year - 1980
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1980.tb00079.x
Subject(s) - intraperitoneal injection , macrophage , exudate , cell , andrology , microbiology and biotechnology , staining , chemistry , glucan , biology , pathology , endocrinology , medicine , biochemistry , in vitro
The effect of a single intraperitoneal injection of yeast glucan in C 3 D 2 mice was studied. The optimal dose for the induction of a cellular exudate was 0.4 mg/kg, producing 17.6 times 10 6 cells on day 7 after injection. The number of T‐cells, B‐cells and macrophages in this exudate was analysed by the use of morphology, immunofluorescence and the non‐specific esterase staining. An increase in both T‐cells, B‐cells and macrophages was found after glucan injection. There was an 8‐fold increase in macrophages, but the increase lasted only 14 days. The increase in T‐cells was smaller, but lasted at least 35 days. The effect of pretreatment with glucan on the development of an inoculated syngeneic methylcholanthrene induced sarcoma was studied. No effect on tumour take could be demonstrated, but differences in T‐cell, B‐cell and macrophage content of the developing tumour were found. A striking feature was a prolonged increase in tumour T‐cell content.