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Haemagglutination by Staphylococcus Saprophyticus And Other Staphylococcal Species
Author(s) -
Hovelius Birgitta,
Mårdh Peranders
Publication year - 1979
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1979.tb02401.x
Subject(s) - staphylococcus saprophyticus , hemagglutination , microbiology and biotechnology , agglutination (biology) , staphylococcus , staphylococcus aureus , biology , bacteria , virology , antigen , immunology , virus , genetics
Staphylococcus saprophyticus was found to differ from Staphylococcus epidermidis and Staphylococcus aureus by its ability to agglutinate sheep erythrocytes. On testing 30 strains of each species, 28 strains of S. saprophyticus and one strain each of the other two species, caused agglutination. Twenty‐eight of 30 strains of Staphylococcus cohnii and Staphylococcus xylosis failed to cause haemagglutination. The haemagglutinating activity of S. saprophyticus. when using a 10 per cent bacterial suspension was demonstrated in dilutions of 1:2–1:32. It was reduced twofold, at most, when exposing the bacteria to 56° C for 30 minutes, while no agglutination could be demonstrated after treatment for 10 minutes at 8° C. No haemagglutination could be demonstrated after treatment of the bacteria with a 5 per cent solution of trypsin. Treatment of S. saprophyticus with 0.1 M EDTA did not affect the haemagglutinating activity, whereas exposure of the bacteria to 10 per cent trichloroacetic acid reduced the activity. The haemagglutination was D‐mannose‐resistant, and it was inhibited by homologous rabbit antiserum. The agglutinates dispersed when heated at 45–56°C for 30 minutes. A few of the strains of S. saprophyticus tested also agglutinated human, bovine, and guinea pig erythrocytes.