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Diagnosis of Salmonella Bacteria: Antibodies Against Synthetic Salmonella O‐Antigen 8 for Immunofluorescence and co‐Agglutination using Sensitized Protein A‐Containing Staphylococci
Author(s) -
Svenungsson Bo,
Lindberg ALF A.
Publication year - 1979
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1979.tb02399.x
Subject(s) - salmonella , antiserum , microbiology and biotechnology , bacteria , agglutination (biology) , antigen , antibody , direct agglutination test , immunofluorescence , biology , titer , immunology , genetics , serology
An antiserum against the synthetic disaccharide abequose 13 rhamnose (AR), representative of Salmonella O‐antigen 8, coupled to bovine serum albumin (BSA) was used for diagnosis of Salmonella bacteria by indirect immunofluorescence (IFL) and by co‐agglutination (COA) using sensitized protein A‐containing staphylococci. Among the 1150 enteric bacteria tested in IFL, the antiserum correctly identified all 99 Salmonella serogroup C 2 and C 3 bacteria with O‐antigen 8. No fluorescence was seen with 484 Salmonella bacteria belonging to other serogroups or 567 non‐ Salmonella enteric bacteria. The anti‐AR‐BSA serum was favourable as compared to a conventional Salmonella factor O8 serum as regards both titre and specificity. In the COA test, all 22 Salmonella serogroup C 2 and C 3 strains agglutinated strongly and within seconds, whereas no agglutination could be seen when 93 Salmonella bacteria representing other serogroups were tested.