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DIFFERENCE IN GROWTH OF HORMONE DEPENDENT AND HORMONE INDEPENDENT MAMMARY TUMOURS OF GR MICE, IN VIVO AND IN VITRO
Author(s) -
BRIAND PER,
THORPE SUSAN M.,
DAEHNFELDT JOHAN L.
Publication year - 1979
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1979.tb00073.x
Subject(s) - in vivo , in vitro , doubling time , biology , hormone , cell culture , thymidine , cell growth , neoplasm , mammary gland , cell , endocrinology , medicine , pathology , cancer , biochemistry , genetics , breast cancer
Growth in vivo of hormone dependent (HD) and hormone independent (HI) GR mouse mammary tumours was evaluated on the basis of doubling time (t d ) and TD 50 , i.e. the number of tumour cells required to produce a tumour in 50% of inoculated animals within 2 months. TD 50 was 10–100 times higher for HD than for HI tumour cells whereas t d was the same for the two types of tumours. By explanting minced tumour tissue in a modified MEM medium supplemented with 10–20% fetal bovine serum and insulin (5 μg/ml), monolayer cultures consisting of islands of epithelial cells were obtained. Sixty‐five per cent of primary cultures from 71 tumours were tumourigenic as tested by reinoculation into mice. Nearly all the tumourigenic cultures had retained the hormone dependence or independence of the original tumour. In order to compare the growth of HD and HI tumours in vitro , 6 primary tumours were transplanted serially to follow the progression from HD to HI growth. Cell proliferation in vitro as measured by 3 H‐thymidine incorporation tended to be higher in cultures of HI carcinomas than in cultures of HD carcinomas. The ability of cultures to grow in repeated subcultivations was correlated to the number of transplant generations rather than to the transition from HD to HI growth in vivo.