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RELEASE OF INSULIN IN VITRO FROM NORMAL AND DUCT‐LIGATED RAT PANCREAS
Author(s) -
ANDERSSON ARNE,
HALLBERG ANDERS,
HELLERSTRÖM CLAES,
HULTQUIST GÖSTA,
JANSSON LEIF
Publication year - 1979
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1979.tb00054.x
Subject(s) - pancreas , ligation , insulin , medicine , endocrinology , pancreatic duct , connective tissue , atrophy , in vitro , stimulation , islet , duct (anatomy) , chemistry , biology , anatomy , pathology , biochemistry
Ligation of the pancreatic duct causes atrophy of the acinar cells but leaves the endocrine tissue intact. In the present study, a partial ligation of the pancreatic duct was performed in the rat, and the in vitro insulin response to glucose was compared from both the atrophic and non‐atrophic portions of the pancreas. Subsequent morphological studies of the duct‐tied portion of the pancreas indicated a complete lack of acinar cells and a possible neoformation of ducts, fat cells and connective tissue. However, islets were present in normal amounts and appeared well preserved. Measurements of the insulin release in vitro from this ligated pancreatic tissue showed that an increase of the glucose concentrations from 3.3 to 16.7 mmol/l resulted in a six‐fold stimulation. A further two‐fold stimulation was seen after addition of theophylline to the high‐glucose incubation medium. In addition, only under the latter conditions was there a significantly increased insulin release from both the nonatrophic portion of the pancreas and from the pancreas of sham‐operated animals. It is concluded that duct ligation does not diminish the glucose sensitivity of rat islet B‐cells. Thus, the present study does not support the view proposed previously that islet tissue is functionally of a foetal nature following duct ligation.