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DENSITY DETERMINATIONS OF HUMAN PARATHYROID GLANDS BY DENSITY GRADIENTS
Author(s) -
ÅKERSTRÖM GÖRAN,
PERTOFT HÅKAN,
GRIMELIUS LARS,
JOHANSSON HENRY
Publication year - 1979
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1979.tb00029.x
Subject(s) - percoll , density gradient , chemistry , aqueous solution , ficoll , chromatography , analytical chemistry (journal) , mineralogy , centrifugation , biochemistry , geology , peripheral blood mononuclear cell , oceanography , in vitro
The densities of human parathyroid glands were measured in density gradient columns of various media. Percoll (an aqueous colloidal solution of polyvinylpyrrolidone‐coated silica), equilibrated to 300 mOsm with sodium chloride, was found to be the ideal gradient medium for density measurements of tissues with a density > 1.0 g/ml, which includes most parathyroid glands. The densities of the glands varied between 0.96 and 1.06 g/ml. Density measurements in gradients of Percoll were simple and reproducible and were made with an accuracy of approximately 0.001 g/ml. In other gradient media (aqueous solutions of sucrose and Ficoll, and organic solvents) there was a drift in density caused by osmotic or lipid‐solving effects of the media. For measurements of densities <1.0 g/ml no ideal gradient medium was found, but a silicon oil or carbon tetrachloride/kerosene gradient could be used with somewhat reduced accuracy. It is concluded that the density gradient technique with the use of Percoll is potentially useful as a complement to routine histopathological parathyroid diagnosis, as glandular density is a good indicator of the relative proportions of parenchymal and fat tissue.