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EVIDENCE FOR NON‐SPECIFICALLY BOUND IgG IN HUMAN TUMOURS
Author(s) -
Wesenberg Finn,
TöNder Olav
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1978.tb02588.x
Subject(s) - antibody , immunoglobulin g , pathology , specific antibody , chemistry , microbiology and biotechnology , biology , immunology , medicine
Heat eluates of homogenized human malignant and normal tissues were prepared using the continuous flow elution technique. IgG antibodies to rabbit erythrocytes served as marker antibodies for non‐specifically bound IgG. Eluates of all of the 13 malignant tumours tested contained IgG. Most were eluted at 56° C., but considerable amounts also at 37° C and 45° C. Marker antibodies were present in eluates of 4 of the tumours, indicating that at least parts of the IgG associated with malignant human tumours are non‐specifically bound. Eight tumours contained too little IgG to detect marker antibodies, and one contained high amounts of IgG without marker antibodies. Eluates of normal organs contained either no or only small amounts of IgG, although apparently normal kidneys from older individuals contained some more IgG. Marker antibodies were detected in some of the eluates indicating nonspecific binding.