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IMMUNOCHEMISTRY OF SALMONELLA O‐ANTIGENS:
Author(s) -
Carlsson Hans Erik,
Lindberg Alf A.
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1978.tb02586.x
Subject(s) - antibody , hapten , antigen , bovine serum albumin , immunochemistry , chemistry , salmonella , hemagglutination , immunoglobulin m , microbiology and biotechnology , antiserum , immunization , immunoglobulin g , biology , biochemistry , immunology , bacteria , genetics
Immunization of rabbits with the synthetic disaccharide‐protein conjugate, tyvelose 1→3 mannose 1→ bovine serum albumin (TM‐BSA) in Freund's complete adjuvant, gave rise to antibodies directed against both the disaccharide hapten and the carrier protein. The hapten antibodies were specific for O‐antigen 9 of Salmonella and were superior in terms of specificity to conventional O factor 9 antibodies, as judged by passive haemagglutination and enzyme‐linked immunosorbent assay (ELISA). A complement dependent bactericidal assay demonstrated that the TM‐BSA antibodies had bacteriolytic activity also directed against the O‐antigen 9 of Salmonella . The high specificity was evident in both the IgG and IgM antibody classes. In the bactericidal assay the IgM antibodies were more active than antibodies of the IgG class. The results suggested that IgG antibodies in hyperimmune serum blocked the complement dependent bactericidal activity of IgM antibodies.