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LOCALIZATION OF Fc RECEPTORS IN HUMAN AND RAT MALIGNANT TISSUES
Author(s) -
TöNder Olav,
Krishnan Engikolai C.,
Morse Paul A.,
Jewell William R.,
Humphrey Loren J.
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1978.tb02576.x
Subject(s) - receptor , differential centrifugation , centrifugation , cell , antibody , fc receptor , cell surface receptor , membrane , cell membrane , chemistry , cell culture , microbiology and biotechnology , biology , biochemistry , immunology , genetics
Three human malignant tumors and a rat carcinoma (Walker 256), which all showed strong Fc receptor activity in tests with sheep erythrocytes (E) sensitized with rabbit IgG antibodies (A), were used to study the distribution of the receptor in subcellular fractions isolated by differential centrifugation. Smears of the three crude sediments obtained (nuclei, mitochondria rich and membrane rich) all possessed receptor activity. EA incubated in the suspension of cell fractions did not attach to Fc receptor positive tissue sections or to cells in suspension. Cells from which the surface receptors had been eluted still showed activity in sections of cell pellets. Apparently the Fc activity is connected with both external and internal membranes.