Premium
Freezing Of Rat Lymphocytes
Author(s) -
Hem Einar
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1978.tb02573.x
Subject(s) - spleen , thymidine , macrophage , lymphocyte , microbiology and biotechnology , cell , biology , chemistry , immunology , in vitro , biochemistry
Spleen lymphocyte proliferation, as measured by 3 H‐thymidine incorporation induced by phytohae‐magglutinin (PHA), was increased after freeze‐thawing with 10% dimethyl sulphoxide. Depletion or intoxication of macrophages in fresh spleen cell preparations also increased lymphocyte proliferation in response to PHA. On the other hand, freezing of macrophage‐depleted spleen cell suspensions lowered 3 H‐thymidine uptake of stimulated cultures. At concentrations above 3%, macrophages added to cultures of fresh purified lymphocytes showed a dose‐dependent inhibitory effect on the PHA response, and fresh macrophages were more inhibitory than frozen‐thawed macrophages. Purified lymphocytes mixed with 10% macrophages showed a higher response after freeze‐thawing. It is concluded that macrophages suppress the lymphoproliferative response to PHA in rat spleen cell cultures, and that these macrophages are more sensitive than lymphocytes to the present freeze‐thaw process.