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LEUKOCYTE CHEMOTACTIC ACTIVITY IN CULTURES OF UNSTIMULATED HUMAN LYMPHOCYTES
Author(s) -
Arvilommi H.,
Laatikainen Aino,
RäSäNen Liisa
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1978.tb02559.x
Subject(s) - chemotaxis , lymphokine , in vitro , chemistry , biological activity , microbiology and biotechnology , biology , immunology , biochemistry , receptor
We have shown earlier that unstimulated human lymphocytes in in vitro cultures produce migration inhibitory factor into the supernatant. The evidence of spontaneous lymphokine synthesis is strengthened further by this study, which demonstrates leukocyte chemotactic activity in these culture supernatants. The factor has a molecular weight of more than 5000 daltons, it resisted heating for 15 min at 100°C., and showed maximum activity at dilution 1:4–1:8 of the supernatants.