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Demonstration of Hepatitis B‐Surface Antigen in Liver Biopsies
Author(s) -
Clausen Per P.,
Thomsen Per
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1978.tb02060.x
Subject(s) - orcein , immunoperoxidase , staining , pathology , antigen , hepatitis , biology , microbiology and biotechnology , chemistry , antibody , medicine , virology , immunology , monoclonal antibody
Staining of 1000 consecutive liver biopsies with orcein showed positive reaction in the cytoplasm of ground‐glass hepatocytes in 18 of the biopsies. On new sections of the 18 orcein positive biopsies immunoperoxidase staining was performed in order to demonstrate hepatitis B‐surface antigen (HB s Ag). After destaining the same sections were stained with orcein. All biopsies showing positive orcein staining showed positive immunoperoxidase reaction. The number of positive cells was in biopsies with less than 20 positive cells per mm 2 biopsy larger using the immunoperoxidase staining than with orcein staining. Further the staining contrast was more pronounced. Immunoperoxidase staining thus seems more sensitive than orcein staining for demonstrating HB s Ag in liver tissues. Orcein stains HB s Ag in liver tissue even though the antigen determinants are blocked by antibodies.