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The Effect of Tobacco Smoke Condensate on the Growth and Longevity of Human Diploid Fibroblasts
Author(s) -
Litwin J.,
Enzell C.,
Pilotti A.
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1978.tb02025.x
Subject(s) - longevity , cigarette smoke , ploidy , chemistry , wi 38 , carcinogen , nicotine , fraction (chemistry) , in vitro , cell growth , tobacco smoke , cell , toxicology , biology , biochemistry , chromatography , genetics , organic chemistry , neuroscience , gene
Human embryonic diploid lung fibroblasts were exposed to various fractions of cigarette smoke condensate over their in vitro life‐time. Most fractions were toxic at a concentration of 50 μg/ml, with the exception of the strong acid and one water soluble basic fraction, which stimulated growth and increased longevity significantly at this concentration. Most fractions produced no effect on cell growth at 10 μg/ml, with the exception of another basic fraction which inhibited growth at 1 μg/ml. Nicotine had no apparent effect on growth and longevity at 50 μg/ml. The neutral fraction containing the polynuclear hydrocarbon carcinogens produced normal growth and longevity at 10 μg/ml. No cell transformations were observed.