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LABORATORY IDENTIFICATION OF PATHOGENIC NEISSERIA WITH SPECIAL REGARD TO ATYPICAL STRAINS: AN EVALUATION OF SUGAR DEGRADATION, IMMUNOFLUORESCENCE AND CO‐AGGLUTINATION TESTS
Author(s) -
OLCÉN PER,
DANIELSSON DAN,
KJELLANDER JAN
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1978.tb00052.x
Subject(s) - neisseria , microbiology and biotechnology , neisseria meningitidis , sugar , neisseria gonorrhoeae , immunofluorescence , false positive paradox , serology , agglutination (biology) , maltose , bacteria , biology , antibody , biochemistry , sucrose , immunology , genetics , machine learning , computer science
Sugar degradation tests (SDT) were compared with immunofluorescence (IFL) and co‐agglutination (COA) tests for the diagnosis of Neisseria gonorrhoeae (GO and Neisseria meningitidis (MC). Somewhat more than 5% of the GC strains and 8% of the MC strains were misinterpreted by SDT. On most occasions the disagreement between SDT and serological tests was due to the inability of the MC strains (less so for GC strains) to degrade sugars correctly. Because of this, three out of 15 strains (20%) from pharyngeal specimens were primarily considered to be GC by SDT but were identified as MC by COA tests. Deficiencies in sugar degradations were also found in a group of clinical problem strains. Many of them were unable or had a decreased ability to degrade glucose or maltose but were diagnosed distinctly as MC by the COA test. There were no false positives with the IFL or COA tests, but 2% of the GC strains and 26% of the MC carrier strains (non‐groupable) were not identified by COA. Both IFL and COA tests are good adjuncts to SDT for the diagnosis of GC and clinically significant MC, since the results are reliable and the tests rapid and simple to perform.