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CHLAMYDIA TRACHOMATIS FROM MEN WITH NON‐GONOCOCCAL URETHRITIS. SIMPLIFIED PROCEDURE FOR CULTIVATION AND ISOLATION IN REPLICATING McCOY CELL CULTURE
Author(s) -
Csángó Peter A.
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1978.tb00041.x
Subject(s) - chlamydia trachomatis , urethritis , centrifugation , non gonococcal urethritis , isolation (microbiology) , microbiology and biotechnology , chlamydia trachomatis infection , chlamydia , gonococcal infection , virology , chlamydiae , biology , medicine , chemistry , immunology , chromatography , sexually transmitted disease , human immunodeficiency virus (hiv) , syphilis
Chlamydia Irachomatis was cultivated on replicating McCoy cells without the addition of antimetabolites. A further technical modification was centrifugation of the specimens at room temperature at 4000 g, thus making it possible to use the method in any microbiological laboratory. C. trachomatis was isolated from 36 of 81 patients (44.4%) with non‐gonococcal urethritis. This rate compares well with reported isolation rates using antireplicative agents and higher centrifugation temperatures.